Our Abpromise guarantee covers the use of ab205921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Please refer to protocol section here.
For antigen buffer for FFPE tissue, it is recommended to use Universal HIER antigen retrieval reagent (ab208572).
Our Abpromise guarantee covers the use of ab205921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Please refer to protocol section here.
For antigen buffer for FFPE tissue, it is recommended to use Universal HIER antigen retrieval reagent (ab208572).
Our Abpromise guarantee covers the use of ab205921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Please refer to protocol section here.
For antigen buffer for FFPE tissue, it is recommended to use Universal HIER antigen retrieval reagent (ab208572).
Our Abpromise guarantee covers the use of ab205921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Please refer to protocol section here.
For antigen buffer for FFPE tissue, it is recommended to use Universal HIER antigen retrieval reagent (ab208572).
Our Abpromise guarantee covers the use of ab205921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Please refer to protocol section here.
For antigen buffer for FFPE tissue, it is recommended to use Universal HIER antigen retrieval reagent (ab208572).
Description
Molecular Probes™ Antibody Conjugate Purification kits efficiently purify antibody conjugates from unconjugated dye or biotin. This particular kit is optimized for the purification of 20–50µg of antibody conjugate. Purification is accomplished using an easy-to-assemble size-exclusion spin column containing BioGel™ P-6 fine resin. Each kit contains sufficient material for 3 separate purifications, and can also be used to purify proteins with a molecular weight greater than 12 kDa away from unconjugated labels.
Additional Features of this Antibody Conjugate Purification Kit:
· Purified conjugate ready-to-use in ~10 minutes with ~5 minutes of hands-on time
· Designed for low-molecular weight labels (<3000 Da, e.g. Alexa Fluor™ dyes); cannot be used for removing unconjugated high-molecular weight labels (>8 kDa) such as enzymes (HRP) or proteins (R-phycoerythrin)
More about Protein and Antibody Labeling
We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental set-up. To learn more about our labeling kits, see Kits for Labeling Proteins and Nucleic Acids in The Molecular Probes™ Handbook or visit our antibody labeling page.
