Our Abpromise guarantee covers the use of ab20S921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

IHC-P

Use a concentration of 2 Qg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Please refer to protocol section here.

For antigen buffer for FFPE tissue, it is recommended to use Universal HIER antigen retrieval reagent (ab208572).

Description

Molecular Probes™ Antibody Conjugate Purification kits efficiently purify antibody conjugates from unconjugated dye or biotin. This particular kit is optimized for the purification of
20-50Qg of antibody conjugate. Purification is accomplished using an easy-to-assemble size-exclusion spin column containing BioGel™ P-6 fine resin. Each kit contains sufficient
material for 3 separate purifications, and can also be used to purify proteins with a molecular weight greater than 12 kDa away from unconjugated labels.

Additional Features of this Antibody Conjugate Purification Kit:

• Purified conjugate ready-to-use in -10 minutes with -5 minutes of hands-on time
• Designed for low-molecular weight labels (<3000 Da. e.g. Alexa Fluor™ dyes): cannot be used for removing unconjugated high-molecular weight labels C>8 kDa) such as enzymes
CHRP) or proteins (R-phycoerythrin)

More about Protein and Antibody Labeling

We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental set-up. To learn more about our labeling kits, see
Kits for Labeling Proteins and Nucleic Acids in The Molecular Probes™ Handbook or visit our antibody labeling cage.